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soluble cx3cl1 elisa  (R&D Systems)


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    R&D Systems soluble cx3cl1 elisa
    Figure 3. Interactions of <t>CX3CL1</t> and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.
    Soluble Cx3cl1 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/soluble+cx3cl1+elisa/10__1158_slash_1541___7786__mcr___22___0673-42-1-34?v=R%26D+Systems
    Average 94 stars, based on 34 article reviews
    soluble cx3cl1 elisa - by Bioz Stars, 2026-08
    94/100 stars

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    1) Product Images from "Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling"

    Article Title: Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling

    Journal: Molecular Cancer Research

    doi: 10.1158/1541-7786.mcr-22-0673

    Figure 3. Interactions of CX3CL1 and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.
    Figure Legend Snippet: Figure 3. Interactions of CX3CL1 and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.

    Techniques Used: Expressing, Comparison, Staining, Real-time Polymerase Chain Reaction, Migration, Invasion Assay, MANN-WHITNEY

    Figure 4. Interactions of CX3CL1 and CX3CR1-positive macrophages increase ECM remodeling by regulation of MMP9 expression. A, Comparison of Mmp9 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. B, Western blot results showing regulation of MMP9 expression in Raw264.7 cell line by CX3CL1 treatment with or without CX3CR1 knockdown (top) or AZD8797 (bottom). C, Trans-well invasion assay of Raw264.7 cell line with or without CX3CL1 and MMP9 inhibitor JNJ0966. D, Trans-well invasion assay of 4T1 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media. E, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and AZD8797 treated Raw264.7 conditioned media (arrow: well formed endothelial tube. F, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media (arrow: well formed endothelial tube). Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test.
    Figure Legend Snippet: Figure 4. Interactions of CX3CL1 and CX3CR1-positive macrophages increase ECM remodeling by regulation of MMP9 expression. A, Comparison of Mmp9 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. B, Western blot results showing regulation of MMP9 expression in Raw264.7 cell line by CX3CL1 treatment with or without CX3CR1 knockdown (top) or AZD8797 (bottom). C, Trans-well invasion assay of Raw264.7 cell line with or without CX3CL1 and MMP9 inhibitor JNJ0966. D, Trans-well invasion assay of 4T1 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media. E, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and AZD8797 treated Raw264.7 conditioned media (arrow: well formed endothelial tube. F, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media (arrow: well formed endothelial tube). Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test.

    Techniques Used: Expressing, Comparison, Western Blot, Knockdown, Invasion Assay, Tube Formation Assay, MANN-WHITNEY

    Figure 7. CX3CL1 levels in plasma of patients with breast cancer indicates future development of liver metastasis. A, Soluble CX3CL1 levels in plasma of 96 non-metastasis or 59 distant metastasis breast cancer patients identified by ELISA. B, Comparison of Kaplan-Meier distant metastasis free survival curves according to CX3CL1 levels in plasma of patients with breast cancer (HR; Hazard ratio, CI; Confidence interval). C, Summary of our experimental results. Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test for A and log rank test for B.
    Figure Legend Snippet: Figure 7. CX3CL1 levels in plasma of patients with breast cancer indicates future development of liver metastasis. A, Soluble CX3CL1 levels in plasma of 96 non-metastasis or 59 distant metastasis breast cancer patients identified by ELISA. B, Comparison of Kaplan-Meier distant metastasis free survival curves according to CX3CL1 levels in plasma of patients with breast cancer (HR; Hazard ratio, CI; Confidence interval). C, Summary of our experimental results. Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test for A and log rank test for B.

    Techniques Used: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Comparison, MANN-WHITNEY



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    soluble cx3cl1 elisa  (R&D Systems)
    94
    R&D Systems soluble cx3cl1 elisa
    Figure 3. Interactions of <t>CX3CL1</t> and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.
    Soluble Cx3cl1 Elisa, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/soluble+cx3cl1+elisa/10__1158_slash_1541___7786__mcr___22___0673-42-1-34?v=R%26D+Systems
    Average 94 stars, based on 1 article reviews
    soluble cx3cl1 elisa - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

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    Figure 3. Interactions of CX3CL1 and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.

    Journal: Molecular Cancer Research

    Article Title: Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling

    doi: 10.1158/1541-7786.mcr-22-0673

    Figure Lengend Snippet: Figure 3. Interactions of CX3CL1 and CX3CR1-positive macrophages increase invasion of breast cancer cells. A and B, Correlation of Cx3cr1 and Cx3cl1 mRNA expression in liver and lung of PDX models and non-tumor bearing mice. C, Comparison of Cx3cl1 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. D, IHC about CX3CL1 in 4T1-tumor bearing mice. E, Identification of CX3CL1-positive endothelial cell population a. Immunofluorescence staining against CX3CL1 (green) and F4/80 (red) in liver tissues of non-tumor bearing mouse and 4T1 bearing mouse. co-localized areas were shown as white color. F, Real time PCR of Cx3cl1 in 4T1-tumor bearing mice. G, Trans-well migration assay of Raw264.7 cell line with or without CX3CL1. H, Trans-well invasion assay of siNTC or siCX3CR1 treated Raw264.7 cell line with or without CX3CL1. I, Trans-well invasion assay of 4T1 and EO771 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and CX3CR1 inhibitor AZD8797 treated Raw264.7 conditioned media. Error bars denote mean SD. , P < 0.05; , P <0.01; , P < 0.001. P values are determined by the Mann–Whitney test.

    Article Snippet: The soluble CX3CL1 ELISA was performed with filtered plasma samples of total 155 patients with breast cancer which were composed of 59 metastasis patients and 96 non-metastasis patients by Human CX3CL1/Fractalkine Quantikine ELISA Kit (R&D Systems).

    Techniques: Expressing, Comparison, Staining, Real-time Polymerase Chain Reaction, Migration, Invasion Assay, MANN-WHITNEY

    Figure 4. Interactions of CX3CL1 and CX3CR1-positive macrophages increase ECM remodeling by regulation of MMP9 expression. A, Comparison of Mmp9 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. B, Western blot results showing regulation of MMP9 expression in Raw264.7 cell line by CX3CL1 treatment with or without CX3CR1 knockdown (top) or AZD8797 (bottom). C, Trans-well invasion assay of Raw264.7 cell line with or without CX3CL1 and MMP9 inhibitor JNJ0966. D, Trans-well invasion assay of 4T1 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media. E, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and AZD8797 treated Raw264.7 conditioned media (arrow: well formed endothelial tube. F, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media (arrow: well formed endothelial tube). Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test.

    Journal: Molecular Cancer Research

    Article Title: Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling

    doi: 10.1158/1541-7786.mcr-22-0673

    Figure Lengend Snippet: Figure 4. Interactions of CX3CL1 and CX3CR1-positive macrophages increase ECM remodeling by regulation of MMP9 expression. A, Comparison of Mmp9 mRNA expression in liver and lung tissues of PDX models and non-tumor bearing mice. B, Western blot results showing regulation of MMP9 expression in Raw264.7 cell line by CX3CL1 treatment with or without CX3CR1 knockdown (top) or AZD8797 (bottom). C, Trans-well invasion assay of Raw264.7 cell line with or without CX3CL1 and MMP9 inhibitor JNJ0966. D, Trans-well invasion assay of 4T1 breast cancer cell lines in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media. E, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and AZD8797 treated Raw264.7 conditioned media (arrow: well formed endothelial tube. F, Tube formation assay of HUVEC cells in matrigel pre-conditioned by CX3CL1 and JNJ0966 treated Raw264.7 conditioned media (arrow: well formed endothelial tube). Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test.

    Article Snippet: The soluble CX3CL1 ELISA was performed with filtered plasma samples of total 155 patients with breast cancer which were composed of 59 metastasis patients and 96 non-metastasis patients by Human CX3CL1/Fractalkine Quantikine ELISA Kit (R&D Systems).

    Techniques: Expressing, Comparison, Western Blot, Knockdown, Invasion Assay, Tube Formation Assay, MANN-WHITNEY

    Figure 7. CX3CL1 levels in plasma of patients with breast cancer indicates future development of liver metastasis. A, Soluble CX3CL1 levels in plasma of 96 non-metastasis or 59 distant metastasis breast cancer patients identified by ELISA. B, Comparison of Kaplan-Meier distant metastasis free survival curves according to CX3CL1 levels in plasma of patients with breast cancer (HR; Hazard ratio, CI; Confidence interval). C, Summary of our experimental results. Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test for A and log rank test for B.

    Journal: Molecular Cancer Research

    Article Title: Triple-Negative Breast Cancer-Derived Extracellular Vesicles Promote a Hepatic Premetastatic Niche via a Cascade of Microenvironment Remodeling

    doi: 10.1158/1541-7786.mcr-22-0673

    Figure Lengend Snippet: Figure 7. CX3CL1 levels in plasma of patients with breast cancer indicates future development of liver metastasis. A, Soluble CX3CL1 levels in plasma of 96 non-metastasis or 59 distant metastasis breast cancer patients identified by ELISA. B, Comparison of Kaplan-Meier distant metastasis free survival curves according to CX3CL1 levels in plasma of patients with breast cancer (HR; Hazard ratio, CI; Confidence interval). C, Summary of our experimental results. Error bars denote mean SD. , P < 0.05; , P < 0.001. P values are determined by the Mann–Whitney test for A and log rank test for B.

    Article Snippet: The soluble CX3CL1 ELISA was performed with filtered plasma samples of total 155 patients with breast cancer which were composed of 59 metastasis patients and 96 non-metastasis patients by Human CX3CL1/Fractalkine Quantikine ELISA Kit (R&D Systems).

    Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Comparison, MANN-WHITNEY